ARUP's Laboratory Test Directory

BRAF codon 600 Mutation Detection by Pyrosequencing : 2002498

Mnemonic: BRAF PCR

Methodology: Polymerase Chain Reaction/Pyrosequencing
Performed: Sun-Sat
Reported: 10-14 days
Specimen Required: Collect: Tumor tissue.

Specimen Preparation: Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Protect from excessive heat. Transport tissue block or 5 unstained 5-micron slides. (Min: 3 slides).

Storage/Transport Temperature: Room temperature or refrigerated. Ship in cooled container during summer months.

Remarks: Include surgical pathology report.

Unacceptable Conditions: Less than 25 percent tumor. Specimens fixed/processed in alternative fixatives (alcohol, Prefer®) or heavy metal fixatives. Decalcified specimens.

Stability (collection to initiation of testing): Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
Interpretive Data: Refer to report.

Refer to Statement B under Testing Information at http://www.aruplab.com.
CPT Code(s): 88381 Microdissection; 83907 Lysis; 83898 Amplification; 83904 Sequencing; 83912 Interpretation and report. Additional CPT code modifiers may be required for procedures performed to test oncologic or inherited disorders.
Cross References: BRAF Gene Mutation Assay, Melanoma (BRAF codon 600 Mutation Detection by Pyrosequencing), BRAF Mutation Analysis, Papillary Thyroid Cancer (BRAF codon 600 Mutation Detection by Pyrosequencing) , Melanoma, BRAF V600 Mutation, Cobas (BRAF codon 600 Mutation Detection by Pyrosequencing)
 
 

 

 

 
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