ARUP's Laboratory Test Directory

Vitamin B6 (Pyridoxal 5-Phosphate) : 0080111

Mnemonic: VIT B6

Methodology: Quantitative High Performance Liquid Chromatography
Performed: Sun-Sat
Reported: 1-4 days
Specimen Required: Patient Prep: Collect specimen after an overnight fast.

Collect: Green (sodium or lithium heparin) or plasma separator tube. Also acceptable: Plain red.

Specimen Preparation: Protect from light during collection, storage, and shipment. Separate plasma from cells ASAP or within 2 hours of collection. Transfer 1 mL plasma to in an ARUP Amber Transport Tube and freeze immediately. (Min: 0.5 mL)

Storage/Transport Temperature: CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.

Unacceptable Conditions: Any specimens other than heparinized plasma or serum. Non-frozen specimens. Serum separator tubes. Specimens collected in EDTA yield a higher pyridoxal 5'-phosphate concentration; therefore, EDTA is not acceptable. Hemolyzed specimens.

Stability (collection to initiation of testing): After separation from cells: Ambient: Unacceptable; Refrigerated: 4 hours; Frozen: 2 months
Reference Interval:
20-125 nmol/L
Interpretive Data: Pyridoxal 5'-phosphate measured in a specimen collected following an 8 hour or overnight fast accurately indicates vitamin B6 nutritional status. Non-fasting specimen concentration reflects recent vitamin intake.
Note: The biologically active form of vitamin B6, pyridoxal 5-phosphate, is measured in this assay.
CPT Code(s): 84207
Cross References: Vitamin B6 (Vitamin B6 (Pyridoxal 5-Phosphate))
 
 

 

 

 
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