ARUP's Laboratory Test Directory

Herpes Simplex Virus DFA with Reflex to Herpes Simplex Virus Culture : 0060280

Mnemonic: HSVFAC

Methodology: Direct Fluorescent Antibody Stain
Performed: Sun-Sat
Reported: Within 24 hours
Specimen Required: Collect: Buccal mucosa lesion, cervical or genital lesion, eye, throat or vesicle swab, bronchoalveolar lavage (BAL), tissue or vesicle fluid.

Specimen Preparation: Fluid: Transfer specimen to a sterile container. Also acceptable: Transfer to viral transport media (ARUP Supply #12884). Available online through eSupply using ARUP Connector contact ARUP Client Services at (800) 522-2787.
Swab or Tissue:
Place in viral transport media. Place each specimen in an individually sealed bag.


Storage/Transport Temperature: Refrigerated.

Remarks: Specimen source preferred.

Unacceptable Conditions: Bone marrow, CSF, or whole blood. Calcium alginate, dry, or wood swabs.

Stability (collection to initiation of testing): Ambient: 4 hours; Refrigerated: 72 hours; Frozen: Unacceptable

Reference Interval:
Negative
Note: If DFA is negative or inadequate, then a HSV culture will be added. Additional charges apply.

Sensitivity of DFA methodology is dependent upon adequacy of the specimen.  If specimen contains fewer than 20 cells, then the DFA result will be reported as "inadequate."
CPT Code(s): 87274 Antigen detection HSV 1; 87273 Antigen detection HSV 2; if reflexed, add 87252 tissue culture; 87253 IP stain.
Cross References: Herpes Simplex Virus by DFA (Herpes Simplex Virus DFA with Reflex to Herpes Simplex Virus Culture), HSV DFA (Herpes Simplex Virus DFA with Reflex to Herpes Simplex Virus Culture)
 
 

 

 

 
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