ARUP's Laboratory Test Directory

Lymphocyte Antigen and Mitogen Proliferation Panel with Cytokine Response to Mitogens, Monokines : 0051587
[ image for: Time Sensitive]
Time Sensitive
  


Mnemonic: LAM MONO

Methodology: Cell Culture/Multiplex Bead Assay
Performed: Tue-Fri
Reported: 9-10 days
Specimen Required: Patient Prep: Collect control specimen from a healthy individual unrelated to patient at approximately the same time as and under similar conditions to the patient.

Collect: Green (sodium heparin) (patient) AND green (sodium heparin) (control). Also acceptable: Yellow (ACD solution A) (patient) AND yellow (ACD solution A) (control). Patient and control specimens must be
collected within 48 hours of test performance.

Specimen Preparation: Transport 20 mL whole blood (patient) AND 20 mL whole blood (control) in original collection tubes. (Min: 14 mL (patient) AND 14 mL (control)) Do not refrigerate or freeze. LIVE CELLS REQUIRED.
Infant Minimum: 3 mL (patient) AND 14 mL (control).

Storage/Transport Temperature: CRITICAL ROOM TEMPERATURE.

Remarks:

Unacceptable Conditions: Yellow (ACD Solution B). Refrigerated or frozen specimens. Specimens in transport longer than 48 hours.

Stability (collection to initiation of testing): Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
Reference Interval:
Available Separately Associated Tests Reference Interval
0096056Lymphocyte Antigen and Mitogen Proliferation Panel By report
0051580Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 1 beta By report
0051581Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 6 By report
0051582Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 8 By report
0051583Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Tumor Necrosis Factor alpha By report

Interpretive Data: Phytohemagglutinin, concanavalin A, pokeweed mitogen, Candida antigen, and tetanus antigen are tested independently in mononuclear cell culture. Cell proliferation in response to mitogens and antigens is determined by 3H-thymidine incorporation. Cytokine production in response to mitogens is determined by multi-analyte fluorescence detection.

Proliferation results are reported as the mitogen and/or antigen stimulated counts per minute (CPM) and a stimulation index (SI) which represents the ratio of the stimulated lymphocyte CPM to an unstimulated control CPM. Cytokine results are reported in pg/mL.

Results are to be used for research purposes or in attempts to understand the pathophysiology of immune, infectious, or inflammatory disorders.

Refer to Statement B under Testing Information at http://www.aruplab.com.
Note: Interpretation comparing the patient results to the simultaneously collected client normal control and the laboratory normal control will be provided by an ARUP medical director.
CPT Code(s): 86353 x 5 Lymphocyte transformation; 83520 x 4 Cytokines
Cross References: IL-1 Beta (Lymphocyte Antigen/Mitogen Prolif. Panel with Cytokine Response to Mitogens, Monokines), IL-6 (Lymphocyte Antigen/Mitogen Prolif. Panel with Cytokine Response to Mitogens, Monokines), IL-8 (Lymphocyte Antigen/Mitogen Prolif. Panel with Cytokine Response to Mitogens, Monokines), TNF Alpha (Lymphocyte Antigen/Mitogen Prolif. Panel with Cytokine Response to Mitogens, Monokines)
 
 

 

 

 
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