Reference Interval:
< 1:20: Not significant
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| Interpretive Data: |
Neutrophil Cytoplasmic Antibodies (C-ANCA = granular cytoplasmic staining, P-ANCA = perinuclear staining) are found in the serum of over 90% of patients with certain necrotizing systemic vasculitides, and usually in less than 5% of patients with collagen vascular disease or arthritis.
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| Note: |
Specimens are screened on ethanol-fixed slides which allow differentiation of C and P patterns. Negatives are reported. Positives are serially diluted and tested on ethanol-fixed slides to establish titer and confirm pattern. All P-ANCA patterns are also tested on formalin-fixed slides to confirm P-ANCAs by removing ANA receptor sites and maintaining cytoplasmic localization of myeloperoxidase or other antigens.
IFA is considered the screening procedure of choice for ANCAs. However, if confirmation of Anti-PR3 or confirmation of Anti-MPO in specimens positive for ANCA is desired, please contact Client Services at (800) 522-2787 to order specific Multi-Analyte Fluorescent Detection (MAFD) testing offered by ARUP. We retain specimens in the laboratory for four weeks and the MAFD tests can usually be performed on the same specimen if sufficient specimen was received and the appropriate test request is added. Refer also to Anti-Neutrophil Cytoplasmic Antibody with Reflex to Titer & MPO/PR3 Antibodies (ARUP test code 2002068).
ANCA antibodies are screened by IFA at 1:20. If the IFA screen is positive, then a titer will be added. Additional charges apply.
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| CPT Code(s): |
86255 ANCA; if positive, add 86256 ANCA titer
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Cross References: |
ANCA (Anti-Neutrophil Cytoplasmic Antibody, IgG), Wegner's Granulomatosis (Anti-Neutrophil Cytoplasmic Antibody, IgG) |