ARUP's Laboratory Test Directory

Kleihauer-Betke Stain for Fetal Hemoglobin : 0040105

Mnemonic: KB

Methodology: Semi-Quantitative Acid Elution Eosin Stain/Microscopy
Performed: Sun-Sat
Reported: Within 24 hours
Specimen Required: Collect: Lavender (EDTA) or pink (K2EDTA).

Specimen Preparation: Transport 3 mL whole blood. (Min: 0.5 mL)

Storage/Transport Temperature: Refrigerated.

Unacceptable Conditions: Frozen specimens. Specimens collected after Rh immunoglobulin administered. Clotted specimens.

Stability (collection to initiation of testing): Ambient: 72 hours; Refrigerated: 72 hours; Frozen: Unacceptable

Reference Interval:
Negative
Interpretive Data: Volume (mL) of fetomaternal hemorrhage (assuming a maternal blood volume of 5 liters) = percent fetal rbcs x50. 

The quantitation of fetomaternal hemorrhage by this method is inherently imprecise.  Therefore, it is important to provide a safety margin when calculating the dose of RhIG. 
Note: This test should be ordered on pregnant or post-partum women who need to be assessed for RhoGAM® or fetal-maternal bleeds. For routine fetal hemoglobin (Hb F) testing, please order Hemoglobin Evaluation with Reflex to Electrophoresis and/or HPLC (0050610).
CPT Code(s): 85460
Cross References: Fetal Hemoglobin, Semi-Quantitative (Kleihauer-Betke Stain for Fetal Hemoglobin)
 
 

 

 

 
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