| Collect: | Three Cellestis QuantiFERON-TB Gold In-Tube collection tubes (nil, TB, and mitogen, ARUP supply #45112) should be used. High-altitude collection tubes (ARUP supply #46261) should be used for elevations between 3,350 and 6,150 feet. A syringe is recommended when collecting blood at elevations between 2,650 and 3,350 feet, or above 6,150 feet. Supplies are available online through eSupply using ARUP Connect™ or by contacting ARUP Client Services at (800) 522-2787. |
QuantiFERON-TB Gold In-Tube Instructional Videos |
| Transport: | Original collection tubes, after incubation and centrifugation, at 2–8°C. | |
| Pediatric Collect/Transport: | Original collection tubes, after incubation and centrifugation, at 2–8°C. | |
| Remarks: | Tubes should be between 17ºC and 25ºC (63ºF to 77ºF) at the time of blood filling. Immediately after filling tubes, shake them 10 times just firmly enough to ensure that the entire inner surface of the tube is coated with blood to solubilize antigens on tube walls. Overenergetic shaking may cause gel disruption and could lead to aberrant results. After mixing, incubate upright at 37°C for 16–24 hours (within 16 hours of collection). If the blood is not incubated immediately after collection, remixing of the tubes by inverting 10 times must be performed immediately prior to incubation. After incubation, centrifuge for 15 minutes at 2000–3000 RCF (g). |
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| Unacceptable Conditions: | Room temperature specimens; specimens that have not been centrifuged. | |
| Stability: | After incubation and centrifugation—ambient: two hours; refrigerated: four weeks; frozen: four months. |
CORP-APPEND-0249J, Rev 2